Genetic marker analysis experiments rely on the detection of changes in the length of a specific DNA fragment to indicate the presence or absence of a genetic marker. Our DNA Sequencing team offers a fast and reliable automated DNA fragment analysis service where fluorescently labeled DNA fragments are electrophoretically separated using the ABI3730xl Genetic Analyzer.
For selected applications (Microsatellite and MLPA), we also offer the final data interpretation as an add-on service for samples labeled with 6-FAM. We perform quality DNA sizing, allele calling, and relative quantitation using the GeneMapper v6.0 analysis software package.
Accurate size calling, consistent band intensities, and low run-to-run migration variations by electrophoresis on ABI 3730xl DNA Analyzer
Sizing to within 1 bp resolution for fragments in the approximate size range of 50 bp to 500 bp
Supported dye labels: 6-FAM (blue), NED (yellow), PET (red), VIC (green)
Our instrument is calibrated for use with Dye Set DS-33 (filter set G5) and will not
work optimally with other dyes.
Multiplex option (up to 4 fluorescent dyes)
Sizing by comparison with GeneScan 500 LIZ Size Standard
Standard size marker (orange dye label) is loaded with each sample.
On-site data interpretation (Microsatellite and MLPA applications only!) using
GeneMapper v6.0 analysis software (add-on service option)
Please note: Data interpretation of NED, PET, VIC, and multiplexed samples are the responsibility of the user. The core will analyze data when samples are labeled with 6-FAM only.
Direct email notification upon service completion
Secure website access to fragment analysis results
Prompt technical support and consultation
Daily (Mon-Fri) processing and free sample pickup at our remote sample drop-off locations
Turnaround time: 24 hours or less
Supported DNA Fragment Analysis Applications:
We are performing our standard fragment analysis services utilizing an ABI 3730xl protocol that has been specifically developed and optimized for Microsatellite and MLPA applications.
If you are interested in another application, please contact us to inquire about technical feasibility.
Additional Fragment Analysis Applications:
Please refer to our Fragment Analysis Guidelines prior to submitting samples.
If you should require any assistance or have questions regarding sample submission or setting up an account with us, please contact us.