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DNA Fragment Analysis

 

Genetic marker analysis experiments rely on the detection of changes in the length of a specific DNA fragment to indicate the presence or absence of a genetic marker. Our DNA Sequencing team offers a fast and reliable automated ready-to-run DNA fragment analysis service where fluorescently labeled DNA fragments are electrophoretically separated using the ABI3730xl Genetic Analyzer.

Fragment Analysis Chromatogram Image

For selected applications (Microsatellite and MLPA), we also offer the final data interpretation as an add-on service. We perform quality DNA sizing, allele calling, and relative quantitation using the GeneMapper v4.0 analysis software package.

Key Features

  • Accurate size calling, consistent band intensities, and low run-to-run migration variations by electrophoresis on ABI 3730xl DNA Analyzer

  • Sizing to within 1 bp resolution for fragments in the approximate size range of 50 bp to 500 bp

  • Supported dye labels: 6-FAM (blue), NED (yellow), PET (red), VIC (green)

    • Our instrument is calibrated for use with Dye Set DS-33 (filter set G5) and will not work optimally with other dyes.

     

  • Multiplex analysis option (up to 4 fluorescent dyes)

  • Sizing by comparison with GeneScan 500 LIZ Size Standard

    • Standard size marker (orange dye label) is loaded with each sample.

     

  • On-site data interpretation (Microsatellite and MLPA applications only!) using GeneMapper v4.0 analysis software (add-on service option)
    Please note: Data interpretation of multiplexed samples are responsibility of the user!

  • Direct email notification upon service completion

  • Secure website access to fragment analysis results

  • Prompt technical support and consultation

  • Daily (Mon-Fri) processing and free sample pickup at our remote sample drop-off locations

  • Turnaround time: 24 hours or less

 

Supported DNA Fragment Analysis Applications:

  • Microsatellite Analysis (short tandem repeats (STRs), variable nucleotide tandem repeats (VNTRs))
  • MLPA Analysis (multiplex ligation-dependent probe amplification)

We are performing our standard fragment analysis services utilizing an ABI 3730xl protocol that has been specifically developed and optimized for Microsatellite and MLPA applications.

If you are interested in another application, please contact us to inquire about technical feasibility.

Additional Fragment Analysis Applications:

  • AFLP Analysis (amplified fragment lengths polymorphisms)
  • Electrophoresis of fluorescent PCR amplicons
  • T-RFLP Analysis (terminal restriction length polymorphism)
  • Single Nucleotide Polymorphism Genotyping (SNP)
  • Relative Fluorescent Quantitation (Loss of Heterozygosity, LOH)

Please refer to our Fragment Analysis Guidelines prior to submitting samples.

If you should require any assistance or have questions regarding sample submission or setting up an account with us, please contact us.