Complete Plasmid Sequencing
In collaboration with Dr. Brian Seed and researchers from his group at the CCIB,
our team has developed a low-cost high-throughput approach for complete plasmid
sequencing using Next-Generation sequencing technology in combination with automated sequence assembly.
Provided by members of our experienced Sequencing and Automation teams, our new service offers
many advantages over conventional primer walking, including deep sequencing coverage and
highest possible data quality at the lowest cost.
We are offering this service on a weekly basis.
Start of Next Service Cycle:
Capacity for nonCOVID-19 samples remains low. Turnaround times have been extended 2-4 weeks while we adhere to social distancing guidelines to keep our staff safe. Thank you for your patience and be well!
Individual samples must be received
at the core facility
no later than 4 pm on Wednesday,
. If you are using one of our dropbox locations for sample delivery, please place the order in the cooler by Wednesday mid-morning to ensure timely delivery.
If you should miss the deadline or if the total customer order volume exceeds
our current production capacity, your samples will be entered into the queue and
processed in the subsequent run. Results for all samples processed in our weekly run will
be available within 8 to 9 business days after the start of the service cycle.
Full 96-well plates can be submitted on any business day
and will be processed on a first come, first serve basis. The waiting time
in queue depends on the total volume of plate orders and our processing capacity. Please
at least 5 business days before your sample plate
arrives at our core facility so that we can schedule your project in a timely manner and process it as quickly as possible.
- Complete characterization of unknown plasmids
- Complete validation of known plasmids
- Highly sensitive detection of contaminating DNA sequences
- No reference sequence required
- Independent of sequencing primers
- Cost-effective high-throughput sequencing
- Sample multiplexing using validated indices
- Fast turnaround time
For detailed sample preparation and submission requirements, please visit our Sample Submission Guidelines.
Our Complete Plasmid Sequencing approach is also feasible for full-length sequencing of PCR amplicons and restriction fragments (Complete Amplicon Sequencing)
as well as for Viral Genome Sequencing.
Please contact us to discuss your specific project needs.