To address the emerging needs of the research community,
the CCIB DNA Core is continuously expanding its diverse portfolio of genomics solutions.
In support of the scientific community's enthusiastic embracement of the CRISPR/Cas9 genome editing
technology, we have developed a cost-effective, high-throughput strategy for identifying
CRISPR/Cas-induced mutations via deep sequencing of PCR amplicons.
Individual samples must be received
at the core facility
no later than 4 pm on Wednesday, .
If you should miss the deadline or if the total customer order volume exceeds
our current production capacity, your samples will be entered into the queue and
processed in the subsequent run. Results for all samples processed in our weekly run will
be available within 7 to 8 business days after the start of the service cycle.
Full 96-well plates can be submitted on any business day and will be processed on a first come, first serve basis. The waiting time
in queue depends on the total volume of plate orders and our processing capacity. Please
contact us at least 5 business days before your sample plate
arrives at our core facility so that we can schedule your project in a timely manner and process it as quickly as possible.
Service Features
CRISPR Amplicon Sequencing Details
Our guidelines for amplicon design, sample preparation and submission can be found under the section
Sample Submission.
A short description of our algorithm used to perform CRISPR variant detection can be found
here.
If you should have any additional questions about our CRISPR Sequencing service,
please contact the Core Director.