CRISPR Sequencing

To address the emerging needs of the research community, the CCIB DNA Core is continuously expanding its diverse portfolio of genomics solutions. In support of the scientific community's enthusiastic embracement of the CRISPR/Cas9 genome editing technology, we have developed a cost-effective, high-throughput strategy for identifying CRISPR/Cas-induced mutations via deep sequencing of PCR amplicons.

We are offering this service on a weekly basis.
Start of Next Service Cycle:

The turnaround time for our NGS service is currently 1-2 weeks. We appreciate your patience during these challenging times!

Individual samples must be received
at the core facility no later than 4 pm on Wednesday, . If you are using one of our dropbox locations for sample delivery, please place the order in the cooler by Wednesday mid-morning to ensure timely delivery. If you should miss the deadline or if the total customer order volume exceeds our current production capacity, your samples will be entered into the queue and processed in the subsequent run. Results for all samples processed in our weekly run will be available within 7 to 8 business days after the start of the service cycle.

Full 96-well plates can be submitted on any business day and will be processed on a first come, first serve basis. The waiting time in queue depends on the total volume of plate orders and our processing capacity. Please contact us at least 5 business days before your sample plate arrives at our core facility so that we can schedule your project in a timely manner and process it as quickly as possible.

Service Features

  •   Cost-effective ultra-deep sequencing of amplicons derived from CRISPR-targeted genomic regions
  •   Highly sensitive detection of CRISPR-Cas9 induced mutations
  •   CRISPR variant calling
  •   No need for universal tags on target-specific amplicons
  •   No need for time-consuming cloning steps
  •   Sample multiplexing using validated indices
  •   Highest possible data quality
  •  Fast turnaround time

CRISPR Amplicon Sequencing Details


Our guidelines for amplicon design, sample preparation and submission can be found under the section Sample Submission. A short description of our algorithm used to perform CRISPR variant detection can be found here.
If you should have any additional questions about our CRISPR Sequencing service, please contact the Core Director.