Rapid Plasmid Sequencing: Data Retrieval

Upon completion of a sequencing run, the data enters our analysis pipeline. Researchers will receive an automated email notification as soon as their data can be accessed through our website.

Please note that your data will only be available for three months after it is released! We strongly encourage our users to download their data as soon as they are available. Our data server is only a temporary storage site which does not allow long-term archiving of sequencing data. All Rapid Plasmid Sequencing data generated at our core facility is subject to deletion without notice after three months.

Accessing Your Data:

To access our data server, please log into your account and click on the My Results button. You have the following options to download your data files:

  • Download an uncompressed file *(.seq) with a single concatenated text file (in FASTA format) of all nucleotide sequences generated for the corresponding order.

  • Download a compressed file (*.sit) containing one FASTA format sequence file (*.seq), one EXCEL file (with coverage information), and one FASTQ format file (containing the raw nanopore data) for each sample of the corresponding order. The EXCEL file provides depth and coverage information for each individual plasmid base that is valuable for ascribing a sequence quality to the read at that position. A descriptive example of a coverage file can be downloaded here.

  • Decompression Software:
    To open compressed *.sit files (which are actually .zip files) on your computer, you can use Stuffit Expander (free) or WinZip (trial version). To download, please select the appropriate link below. You can also use the Linux unzip command.

    Stuffit Expander
    WinZip for Windows
    WinZip for Mac

    Please note:
    *.seq files are plain text files containing your sequence in FASTA format and can be opened with any software capable of viewing plain text or FASTA format files (text editor software such as Word, NotePad, etc.). You may also change the file extension from *.seq to *.txt.