Complete Amplicon Sequencing
Next-Generation Sequencing (NGS) provides a highly efficient solution for characterizing PCR products from any source. Our new low-cost high-throughput approach for Complete Amplicon Sequencing is another specialized application within our NGS service portfolio and utilizes our well-established Complete Plasmid Sequencing protocols. Amplicons or DNA fragments (> 400 bp) are fragmented first and are then entering our standard NGS library preparation pipeline, followed by massively parallel sequencing and automated de novo sequence assembly.
We are offering this service on a weekly basis.
Start of Next Service Cycle:
Capacity for nonCOVID-19 samples remains low. Turnaround times have been extended 2-4 weeks while we adhere to social distancing guidelines to keep our staff safe. Thank you for your patience and be well!
Individual samples must be received
at the core facility
no later than 4 pm on Wednesday, . If you are using one of our dropbox locations for sample delivery, please place the order in the cooler by Wednesday mid-morning to ensure timely delivery.
If you should miss the deadline or if the total customer order volume exceeds
our current production capacity, your samples will be entered into the queue and
processed in the subsequent run. Results for all samples processed in our weekly run will
be available within 8 to 9 business days after the start of the service cycle.
Full 96-well plates can be submitted on any business day and will be processed on a first come, first serve basis. The waiting time
in queue depends on the total volume of plate orders and our processing capacity. Please
contact us at least 5 business days before your sample plate
arrives at our core facility so that we can schedule your project in a timely manner and process it as quickly as possible.
- Complete characterization of PCR amplicons and restriction fragments
Suitable for size range from 400 bp to <15 kb
Variant calling for small amplicons (400-600 bp size range only)
No reference sequence required
Independent of sequencing primers
Cost-effective high-throughput sequencing
Sample multiplexing using validated indices
Fast turnaround time
For detailed sample preparation and submission requirements, please visit our Sample Submission Guidelines.
Please contact us to discuss your specific project needs.