Full-Length Amplicon Sequencing
The DNA core now offers a full-length amplicon sequencing service using Oxford Nanopore's long-read sequencing technology. This service differs from our rapid amplicon sequencing service by utilizing a ligation-based, non-fragmenting library preparation protocol, where each sequenced read corresponds to an entire amplicon molecule. As a result, sequence assembly is virtually unnecessary, and the data accurately represent the true molecular sequence.
Reads spanning the entire length of the amplicon are captured and sequenced, making this service ideal for long or complex amplicons, detection of linked variants across a single molecule, identification of structural variants (including insertions, deletions, and rearrangements), and assessment of full-length molecule integrity. Both clonal amplicons and mixed populations of amplicons are accepted.
The delivered data include a consensus sequence in FASTA format for the resolved amplicon(s), raw sequencing reads in FASTQ format, and three EXCEL files with per-base depth and coverage metrics. These coverage data help evaluate uniformity across the amplicon, identify low-coverage or difficult to sequence regions, and assess overall confidence in the consensus sequence.
Samples submitted by Tuesday at 12:00 PM will have data delivered by end of the day on Friday.
Service Features
- Fast turnaround time
- Cost-effective
- Non-fragmenting library preparation
- Complete characterization of unknown amplicons
- Complete validation of known amplicons
- Independent of sequencing primers
- Independent of read assembly
For detailed sample preparation and submission requirements, please visit our Sample Submission Guidelines.
Please contact us to discuss your specific project needs.